Preparation method of a colloidal system of stabilisation and controlled release of royal jelly components for various uses

A preparation method of a colloidal stabilization system and controlled release of royal jelly components, comprising:

• in a first stage fresh royal jelly is kept in a temperature of 5-7° C. and contains 10-hydroxydecenoic acid in a concentration of >1.8% w/w and is then allowed to reach room temperature;
• in a second stage the fresh royal jelly is gradually dissolved, under stirring in a system of solvents which consists of deionized water, in which hydroxypropyl-β-cyclodextrin or β-cyclodextrin or γ-cyclodextrin in a concentration of 1%-22% w/w has been pre-dissolved and either vegetable 1,3-propanediol or glycerol or vegetable butylene glycol in a ratio of vegetable 1,3-propanediol or glycerol or vegetable butylene glycol to water of from 5/95 to 90/10, where the concentration of royal jelly in the solvent system ranges between 0.5% to 6.0% w/w;
• in a third stage the system of royal jelly/cyclodextrin/solvent system is subjected to stirring at 1000 to 3000 rpm for 30 to 90 minutes at a temperature of 20° C. to 26° C.;
• in a fourth stage, the system royal jelly/cyclodextrin/solvent is added to a liposomal system at a rate of 10 ml/sec at a concentration of 1.0-7.5% w/w, after complete addition of which a pH of the system is set in a range 5.0-8.2 and either Disodium Ethylenediaminetetraacetic acid or Tetrasodium Glutamate Diacetate is added at a concentration of 0.1-0.2% w/w the system subjected to stirring at 4000 rpm for 4 hours, to form a mixture;
• in a fifth stage, the mixture is kept in a hermetically sealed container to rest for 24 hours at 5-7° C. and then, the colloidal system is cold filtered through an array of cartridge filters of pore size 5 μm-1 μm-0.45 μm and the pH is retested, and the pH is readjusted to be in the range of 5.0-8.2 if the pH outside the range of 5.0-8.2;
• in a sixth stage, the system is allowed to reach room temperature and a mean hydrodynamic particles diameter is measured which should range between 220 nm to 650 nm with a polydispersity index ranging from 0.25 to 0.68, wherein if the measured mean hydrodynamic particles diameter or polydispersity index values are out of these ranges, the colloidal system is filtered through an array of cartridge filters with pore size 0.45 μm-0.2 μm at room temperature;
• and
• in a final stage, after finding that the mean hydrodynamic diameter is between 220 nm to 650 nm and the polydispersity index is from 0.25 to 0.68, a total content of polyphenols and total content of 10-hydroxydecenoate acid are measured, while continuously a release rate of polyphenols and 10-hydroxydecenoic acid is measured in a buffer at pH 7.2 at 37° C. and the colloidal system is stored in a dark container at a temperature of 5-7° C., and is stable for 1 year.