A method for providing antioxidant, anti-inflammation, anti-UVB, anti-allergy, whitening, moisturizing, and anti-wrinkle effects to a subject, comprising applying a composition containing extracts of the roselle seeds to a skin of the subject, wherein the extracts of the roselle seeds comprise lauric, palmitic, linoleic, oleic, and stearic acids;
- and
- the extracts of the roselle seeds are prepared using a preparation method comprising steps of:
- steeping of:
- extracting a weight ratio of the 12:1 to 20:1 roselle seeds and an ethanol solution with a concentration of 75 to 95%;
- and steeping the roselle seeds in the ethanol solution to obtain a suspension;
- and
- extraction of:
- the suspension being engaged in an extraction of a continuous and rapid oscillation with an ultrasonic energy with a total energy of 200 to 500 W, wherein 1 to 3 hours of the oscillation is one cycle, and a total number of 3 to 5 cycles is lasted, in order to obtain roselle seed crude extracts.