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US 12,456,540 B2Granted
Systems and methods for identifying and expressing gene clusters
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsBrian Thomas Naughton, Colin Harvey, Ulrich Schlecht, Maureen Elizabeth Hillenmeyer, Joe Horecka
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Abstract
Methods for identifying biosynthetic gene clusters that include genes for producing compounds that interact with specific target proteins are disclosed. Some methods relate to bioinformatics methods for identifying and/or prioritizing biosynthetic gene clusters. Related systems, components, and tools for the identification and expression of such gene clusters are also disclosed.
Claims9
A method for producing a small molecule to screen for inhibition of a first target protein, the method comprising:
- identifying a fungal protein based on its similarity to a first target protein, wherein the fungal protein has at least 60% sequence identity to the first target protein;
- identifying a fungal gene cluster that is within 20,000 bases of a genomic region that encodes the fungal protein;
- expressing the fungal gene cluster or a plurality of genes from the fungal gene cluster in a host cell to produce a compound;
- isolating the compound produced by the fungal gene cluster or the plurality of genes from the fungal gene cluster;
- and
- screening the isolated compound for inhibition of an activity of the first target protein;
- wherein the first target protein is a mammalian protein.
4 more elements
Dependencies are inferred from the claim text.