System, method, computer-accessible medium and apparatus for DNA mapping

A method of mapping nucleotide molecules, comprising:

• incubating a target nucleotide in a magnesium-free mixture, wherein the magnesium-free mixture comprises a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-cellular apoptosis susceptibility (Cas) protein and a guide ribonucleic acid (RNA), and wherein incubating the target nucleotide with the CRISPR-Cas protein binds the CRISPR-Cas protein to the target nucleotide to form a CRISPR-Cas/target nucleotide complex without the CRISPR-Cas protein cleaving the target nucleotide;
• depositing the CRISPR-Cas/target nucleotide complex on a flat surface, wherein after deposition the CRISPR-Cas/target nucleotide complex is bound to the flat surface;
• and
• imaging the CRISPR-Cas/target nucleotide complex on the flat surface by using high-speed atomic force microscopy (AFM) operating in contact mode, wherein the high speed AFM has a probe linear speed of 4 to 10 millimeters per second, wherein the high-speed atomic force microscopy comprises an AFM system which comprises:
• at least one cantilever;
• at least one scanning probe arrangement including a laser positioned over a portion of the at least one cantilever which contacts a surface of at least one sample, wherein a tilt angle of the at least one cantilever with respect to the at least one scanning probe arrangement is less than 10 degrees;
• and a power source configured to generate a displacement noise that is less than 300 Picometers.