US 12,455,288 B2 · Method of isolating and detecting cancer stem cells

Abstract

Disclosed is the in vitro use of at least one lectin which recognises the fucose alpha(1-2) galactose unit for labelling cancer stem cells of organs involved in respiration, in order to obtain labelled cancer stem cells of organs involved in respiration, in a biological sample. In one particular embodiment, the at least one lectin is chosen from the lectins Ulex Europaeus agglutinin 1 (UEA-1) or the homologue thereof, Trichosanthes japonica agglutinin II (TJA-II), Agaricus Bisporus agglutinin (ABA), Amaranthus Caudatus agglutinin (ACA), jacalin, Griffonia Simplicifolia lectin I (GSL-I) and Griffonia Simplicifolia lectin II (GSL-II). In one particular embodiment, the organ involved in respiration is chosen from the lungs, the larynx, the pharynx, the mouth, the nose, the throat, the tongue, the sinuses, the trachea and the saliva glands including the tonsils and the parotid gland.

Claims14

Dependent claims

An in vitro method for isolating cancer stem cells of organs involved in respiration in a biological sample comprising:

(a) a step of labelling the cancer stem cells of organs involved in respiration with at least one lectin recognizing the fucose α 1-2 galactose group, said lectin being conjugated to biotin or a fluorophore, to obtain a biological sample in which the cancer stem cells of organs involved in respiration are labelled with at least one lectin,
followed by
(b) a step of isolating said cancer stem cells of organs involved in respiration labelled with at least one lectin,
said isolation step being carried out via a support functionalized with streptavidin or avidin constituted by magnetic beads and in the presence of a magnet, when said lectin is conjugated to biotin,
and said isolation step being carried out by cell sorting in flow cytometry, when said lectin is conjugated to a fluorophore,
wherein said at least one lectin is chosen from the lectins UEA-1 or its homologue TJA-II, ABA, ACA, jacalin, GSL-I and GSL-II.

3 more elements

Dependencies are inferred from the claim text.

Method of isolating and detecting cancer stem cells

Life of this patent

Method of isolating and detecting cancer stem cells

Life of this patent

Abstract

Claims14