Aminoacyl-tRNA synthetase efficiently introducing lysine derivatives

A mutant protein of aminoacyl-tRNA synthetase, which comprises:

• (1) a deletion mutation of amino acid residue at position 102, positions 128-140, and positions 159-179 in an amino acid sequence corresponding to a wild-type aminoacyl-tRNA synthetase;
• or
• (2) a deletion mutation of amino acid residues at position 102, positions 128-140, and positions 159-179 in an amino acid sequence corresponding to a wild-type aminoacyl-tRNA synthetase by replacing positions 1-184 of the wild-type aminoacyl-tRNA synthetase with another segment of amino acids at positions 1-149 of SEQ ID NO:
• 4;
• and an amino acid mutation of Y384F corresponding to a wild-type aminoacyl-tRNA synthetase;
• or
• (3) on the basis of (2), further mutations of H29Y, D76G, S89G, N91T, R96K, S121P, N129D, S145P and A148T, as related to SEQ ID NO:
• 4;
• or
• (4) on the basis of (2), further mutations of L274A, C313S, and F349Y, as related to SEQ ID NO:
• 4;
• Wherein the amino acid sequence of the wild-type aminoacyl-tRNA synthetase is shown in SEQ ID NO:
• 1 or 2;
• and
• except for the deletion and the mutation, the other amino acids of the mutant protein are the same as the sequence as shown in SEQ ID NO:
• 1 or SEQ ID NO:
• 2.