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AssigneeKt&G Corporation
InventorsHyo Seok Seo, Young Gi Lee, Jeong Heon Lee, Kwang Chul Kim, Woong Hyun Na, Kyoung Hwan Oh, Eun Young Jeon
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Provided are: a plant cell, in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced as compared with a parent cell; a plant having that has reduced heavy metal absorption, and includes the plant cell; a method of reducing heavy metals in a plant, the method including reducing expression or activity of a PCS gene or a protein encoded by the PCS gene, as compared with a parent cell; a CRISPR-Cas9 recombinant vector including a single guide RNA targeting a PCS gene; and a method of preparing a plant with reduced heavy metals, the method including transforming a plant cell with the recombinant vector.
A cell of a Nicotiana species plant having reduced heavy metal absorption in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced by a CRISPR/Cas9 system comprising a single guide RNA(sgRNA) targeting the PCS gene, as compared with a wild-type cell,
A method of reducing heavy metals in a plant of a Nicotiana species, the method comprising reducing expression or activity of a PCS gene or a protein encoded by the PCS gene by a CRISPR/Cas9 system comprising a single guide RNA(sgRNA) targeting the PCS gene, as compared with a wild-type cell of the plant,
Dependencies are inferred from the claim text.