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US 12,454,681 B2Granted
Erythrocyte removal device, mononuclear cell collector, cell culture device, cell culture system, cell culture method, and mononuclear cell collection method
AssigneeI Peace, Inc.
InventorsKoji Tanabe, Ryoji Hiraide, Kenta Suto
Life of this patent
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Abstract
Provided is a cell culture method including introducing a factor into cells in a cell culture vessel, and culturing the cells into which the factor has been introduced in the same cell culture vessel. Also provided is a mononuclear cell collection method including treating blood to prepare a treated blood from which erythrocytes have been at least partially removed, diluting the treated blood, causing sedimentation of mononuclear cells contained in the diluted treated blood, removing the supernatant from the diluted treated blood, and collecting the mononuclear cells.
Claims12
A cell culture method comprising:
- introducing an induction factor into mononuclear cells in a cell culture vessel;
- and
- culturing the mononuclear cells into which the induction factor has been introduced in the same cell culture vessel until iPS cells are induced from the mononuclear cells into which the induction factor has been introduced,
- wherein the cell culture vessel is closed during the introducing and the culturing, and
- gas in the cell culture vessel is not exchanged during the culturing.
2 more elements
Dependencies are inferred from the claim text.