Construction of engineering bacteria for high expression of recombinant human serum albumin
AssigneeShenzhen Protgen Ltd.
InventorsGuodong Chang, Xin Dou, Xiaobo Xiong, Wenchao Wang
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Abstract
Provided is a method for the high expression of a recombinant human serum albumin, characterized in comprising the step of co-expressing: (a) a human serum albumin gene, and (b) one or more rHSA expression promoting factor genes in a yeast host cell.
Claims12
A method for highly expressing recombinant human serum albumin (rHSA) in a yeast host cell, comprising co-expressing a human serum albumin gene, a gene encoding protein disulfide isomerase (PDI), and a gene encoding transcriptional activator HAC1, wherein the yeast host cell is Pichia pastoris.
An engineered yeast that highly expresses recombinant human serum albumin (rHSA) and comprises:
- a human serum albumin gene, a gene encoding PDI, and a gene encoding transcriptional activator HAC1, wherein the yeast is Pichia pastoris, and wherein the gene encoding PDI and the gene encoding transcriptional activator HAC1 are introduced into the engineered yeast.
A method for highly expressing recombinant human serum albumin (rHSA) in Pichia pastoris, comprising co-expressing a gene encoding rHSA, a gene encoding protein disulfide isomerase (PDI), and a gene encoding transcriptional activator HAC1,
- wherein co-expressing rHSA, PDI, and HAC1, increases expression of rHSA relative to co-expressing rHSA and PDI alone.
Dependencies are inferred from the claim text.