Reagent for measuring oxygen concentration in cell and tissue
AssigneeNational University Corporation Gunma University
InventorsToshitada Yoshihara, Tatsuya Hirose, Seiji Tobita
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Abstract
The present invention aims to develop a compound and a reagent having long phosphorescence lifetimes, for use in imaging of a hypoxic cell/tissue or for use in measurement/quantification of the oxygen concentration thereof. The present invention provides a reagent for measuring oxygen concentration, comprising a compound represented by the following General Formula (I) or (II). wherein R1 and R2 each independently represent hydrogen or a C1-C6 hydrocarbon group; and X− represents a counter anion.
Claims10
A method for measuring oxygen concentration, the method comprising administering a compound represented by Formula (I) or (II) to a subject, monitoring a phosphorescence signal of the administered compound, and calculating oxygen concentration from the monitored phosphorescence intensity:
- wherein
- in Formula (II), R1 and R2 each independently represent hydrogen or a C1-C6 hydrocarbon group;
- in Formula (I), R1 and R2 each independently represent a C2-C6 hydrocarbon group;
- and
- X− represents a counter anion.
A method for cancer diagnosis, the method comprising administering a compound represented by Formula (I) or (II) to a subject, monitoring a phosphorescence signal of the administered compound, and detecting as a cancer tissue, a site where an oxygen concentration is decreased relative to an oxygen concentration of normal tissues, from the monitored phosphorescence intensity:
- wherein
- in Formula (II), R1 and R2 each independently represent hydrogen or a C1-C6 hydrocarbon group;
- in Formula (I), R1 and R2 each independently represent a C2-C6 hydrocarbon group;
- and
- X− represents a counter anion.
A compound represented by Formula (I)′ or (II):
- wherein
- R1 and R2 each independently represent hydrogen or a C1-C6 hydrocarbon group;
- R1′ and R2′ each independently represent a C2-C6 hydrocarbon group;
- and
- X− represents a counter anion.
Dependencies are inferred from the claim text.