Cryoprotective compositions, surgical kits, and methods for protection of a surgical site during cryosurgery

A surgical kit for providing a cryoprotective composition configured to be applied during cryotreatment of a patient, the kit comprising:

• at least one first container containing a predetermined amount or volume of at least one biodegradable and/or bioerodible fluid agent comprising a gel and a saline buffer solution;
• and
• at least one second container containing a predetermined amount or volume of at least one non-toxic cryoprotectant agent, the at least one non-toxic cryoprotectant agent comprising a sugar cryoprotectant agent and Dimethyl sulfoxide (DMSO),
• wherein the predetermined amount or volume of the of at least one biodegradable and/or bioerodible fluid agent and the predetermined amount or volume of at least one non-toxic cryoprotectant agent are configured to be mixed together to form the cryoprotective composition in which a therapeutically effective amount of the cryoprotective composition deposited in a body space of the patient in proximity to the cryotreatment remains within at least a portion of the body space for a duration of the cryotreatment, does not cause substantial nerve damage when positioned in the body space for the duration of the cryotreatment, and at least a portion of a body tissue proximate to the body space is viable after the cryotreatment, and
• wherein, when mixed together, the gel comprises from about 1 weight percent to about 10 weight percent of the cryoprotective composition, the sugar cryoprotectant agent comprises from about 5 weight percent to about 10 weight percent of the cryoprotective composition, and the DMSO comprises from about 1 weight percent to about 5 weight percent of the cryoprotective composition.