US 12,453,745 B2 · Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids

Abstract

The present invention relates to the fields of medicine, cell biology, molecular biology and genetics. In particular, the present invention provides methods to isolate and purify microvesicles from cell culture supernatants and biological fluids. The present invention also provides pharmaceutical compositions of microvesicles to promote or enhance wound healing, stimulate tissue regeneration, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth and/or mobility, or alter normal cell growth and/or mobility. The present invention also provides compositions of microvesicles to be used as diagnostic reagents, and methods to prepare the compositions of microvesicles.

Claims24

Dependent claims

A topical composition for treating wounds comprising an aqueous concentrate of a heterogeneous population of purified, intact microvesicles,

wherein the intact microvesicles range in size from 2 nm to 5000 nm,
wherein the intact microvesicles have smooth and uncorrugated borders and appear intact when examined by electron microscopy, and
wherein the intact microvesicles enhance tissue regeneration, promote wound healing, enhance nerve growth, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth, and/or decrease abscess formation of a tissue when administered to a patient.

Dependencies are inferred from the claim text.

Facts

Application

18/332,063Filed 9 Jun 2023 · continuation

Priority

13 Mar 2013

Published

US 2023/0364150 A116 Nov 2023

Granted

28 Oct 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification23 codes

A61K 35/28
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 8/981
Of animal origin: of mammals or bird
A61K 8/983
Of mammals or bird: blood, e.g. plasma
A61K 35/12
Medicinal preparations containing materials or reaction products thereof with undetermined constitution: materials from mammals; Compositions…

Family

Application61/778,591Us provisional application · filed 13 Mar 2013

PCT/US2014/024629Parent of this continuation · filed 12 Mar 2014

US 10,500,231 B2Parent of this continuation · filed 11 Sep 2015

Application16/668,851Parent of this continuation · filed 30 Oct 2019

US 2023/0364150 A1Pre-grant publication · published 16 Nov 2023

US 11,730,768 B2Parent of this continuation

Application14/775,382Parent of this continuation

US 2016/0184363 A1Published application

US 2020/0163999 A1Published application

US 12,453,745 B2This patent

From the US filing history. Foreign family members aren’t listed.

Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids

Life of this patent

Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids

Life of this patent

Abstract

Claims24