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US 12,453,475 B2Granted
Method and device for monitoring fluorescence diffusion images emitted at the surface of a biological tissue and automatically determining the variations between fluorescence images
AssigneeFluoptics
InventorsAnthony Daures, Philippe Rizo
Life of this patent
Estimating the term…
Abstract
A method for monitoring diffusion of a fluorescent marker within a region of biological tissue includes observing the marker's diffusion in a series of images captured by a camera and determining whether to display an image based at least in part on the evolution of the marker's spatial distribution as it perfuses through the tissue.
Claims18
A method for monitoring diffusion of a fluorescent marker within a region of biological tissue, said method comprising:
- using a camera, acquiring fluorescence images from said region, wherein each of said fluorescence images corresponds to a set of pixels, wherein a value of a fluorescence signal is associated with at least certain ones of said pixels, and wherein said value represents an intensity of fluorescence emission from said region;
- during perfusion of said fluorescent marker, measuring diffusion areas, each corresponding to a number of pixels associated with a signal greater than or equal to a predetermined or precalculated signal threshold, in at least two of said acquired fluorescence images, said diffusion areas being areas in which said marker has diffused;
- comparing said diffusion areas by determining a variation, between said at least two of said acquired fluorescence images, in the percentage of the number of pixels associated with said signal greater than or equal to said predetermined or precalculated signal threshold;
- and
- based on said comparison, automatically determining that a change in said number of pixels associated with a signal greater than or equal to said predetermined or precalculated signal threshold, has reached a percentage threshold sufficient for at least a portion of one of said two acquired images to be displayed.
2 more elements
An apparatus for monitoring diffusion of a fluorescent marker within a region of biological tissue, said apparatus comprising:
- a camera for acquiring fluorescence images from said region, wherein each of said fluorescence images corresponds to a set of pixels, wherein a value of a fluorescence signal is associated with at least certain ones of said pixels, and wherein said value represents an intensity of fluorescence emission from said region;
- means for measuring diffusion areas, each corresponding to a number of pixels associated with a signal greater than or equal to a predetermined or precalculated signal threshold, in at least two of said acquired fluorescence images during perfusion of said fluorescent marker, said diffusion areas being areas in which said marker has diffused;
- means for comparing said diffusion areas by determining a variation, between said at least two of said acquired fluorescence images, in the percentage of the number of pixels associated with said signal greater than or equal to said predetermined or precalculated signal threshold;
- and
- means for automatically determining, based on said comparison, that a change in said number of pixels associated with a signal greater than or equal to said predetermined or precalculated signal threshold, has reached a percentage threshold sufficient for at least a portion of one of said two acquired images to be displayed.
2 more elements
Dependencies are inferred from the claim text.