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US 12,453,364 B2Granted
Method for producing a polysaccharide
AssigneeImagine Global Care Corporation
InventorsKazuhisa Sekimizu, Makoto Urai
Life of this patent
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Abstract
A method for producing a polysaccharide includes extracting from broccoli with hot water to obtain an extract, purifying the extract by a chromatography column, and hydrolyzing the purified extract with oxalic acid. The chromatography column can be a DEAE cellulose chromatography column. The polysaccharide produced can have innate immune stimulating activity.
Claims2
A method for producing a polysaccharide, comprising:
- extracting broccoli with hot water to obtain an extract;
- subjecting the extract to ethanol precipitation;
- subjecting obtained precipitates to dialysis and freeze drying to obtain a freeze-dried product;
- subjecting the freeze-dried product to column chromatography;
- collecting peak fractions that give C value (contraction value) of 0.15 or more based on Silkworm Muscle Contraction Assay as purified fractions containing eluted sugars;
- subjecting the peak fractions to dialysis and freeze drying;
- hydrolyzing the freeze-dried peak fractions with oxalic acid to obtain a hydrolyzed product;
- and
- separating the polysaccharide from the hydrolyzed product,
- wherein the polysaccharide has innate immune stimulating activity, and comprises:
- 25 to 45 parts by mole of galacturonic acid, 20 to 50 parts by mole of galactose, 1 to 7 parts by mole of glucose, 0 to 10 parts by mole of arabinose, 0 to 6 parts by mole of xylose, and 4 to 14 parts by mole of rhamnose as constituent sugars;
- and a polygalacturonic acid chain having α-1,4-linked galacturonic acid as a main chain.
9 more elements
Dependencies are inferred from the claim text.