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US 12,448,633 B2Granted
Bacterial N-demthylases as biocatalysts for the production of methylxanthines
AssigneeThe Board of Trustees of The University of Alabama
InventorsRyan M. Summers, Shelby Mills, Meredith Mock
Life of this patent
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Abstract
The present disclosure relates to mutant enzymes and bacterial strains constructed to produce paraxanthine and 7-methylxanthine from caffeine and methods for the manufacture and use thereof.
Claims5
A method of preparing paraxanthine (1,7-dimethylxanthine) and/or 7-methylxanthine, comprising:
- contacting a bacterial strain expressing N-demethylase or a mutant thereof with caffeine, theobromine, or theophylline;
- wherein the bacterial strain also overexpresses frmAB.
Dependencies are inferred from the claim text.