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US 12,448,609 B2Granted
Heterologous expression of short-chain monooxygenases in microorganisms
AssigneeIndustrial Microbes, Inc.
InventorsElizabeth Jane Clarke, Baolong Zhu, Derek Lorin Greenfield, Stephanie Rhianon Jones, Noah Charles Helman
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Abstract
Methods and compositions for the oxidation of short alkanes by engineered microorganisms expressing enzymes are described, along with methods of use.
Claims13
A method for producing diiron monooxygenase, comprising:
- a) culturing a synthetic microorganism comprising:
- (i) at least one synthetic polynucleotide comprising at least one monooxygenase coding region encoding a diiron monooxygenase comprising SEQ ID NO:
- 10, wherein the diiron monooxygenase comprises one or more subunits and
- (ii) at least one protein folding chaperone coding region encoding a protein folding chaperone, each linked to at least one promoter, under suitable culture conditions and for a sufficient period of time to produce the diiron monooxygenase;
- b) lysing the cells, and
- optionally, purifying the diiron monooxygenase to produce a lysate or purified diiron monooxygenase.
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Dependencies are inferred from the claim text.