Recombinant bacteria for producing de-epoxidized epothilone B and use thereof
AssigneesBeijing Biostar Pharmaceuticals Co., Ltd., Chengdu Biostar Pharmaceuticals, LTD.
InventorsLi Tang, Rongguo Qiu
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Abstract
Recombinant Sorangium cellulosum for producing de-epoxidized epothilone B by fermentation, insertional inactivation of an epoK gene in an epothilone biosynthetic gene cluster in the recombinant bacteria, and a method for producing de-epoxidized epothilone B using the recombinant bacteria.
Claims12
A recombinant strain of Sorangium cellulosum for producing de-epoxidized epothilone B via fermentation, in which a cytochrome P450 epoxidase (epoK) gene in an epothilone biosynthesis gene cluster is inactivated, wherein the epoK gene is inactivated by insertion, wherein the inactivated epoK gene comprises the nucleotide sequence of SEQ ID NO:1, and wherein the insertion sequence is from position 291 to position 1960 of SEQ ID NO:1.
Dependencies are inferred from the claim text.