Medium additive for suspension culture, medium composition and culture method
AssigneesDKS Co. Ltd., Tokyo Institute of Technology
InventorsTakeshi Serizawa, Toshiki Sawada, Masahito Nishiura, Rina Murase
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Abstract
To culture cells in a suspended state and easily recover the cultured cells. An additive for suspension culture according to an embodiment is a medium additive which is added to a medium for culturing cells in suspension, the medium additive containing a cellulose oligomer. A medium composition according to an embodiment is a medium composition capable of culturing cells in suspension, the medium composition containing a cellulose oligomer. A cell culture method according to an embodiment includes culturing cells in the medium composition.
Claims13
A cell culture method comprising:
- suspension culturing animal cells in a medium composition comprising a water-insoluble cellulose oligomer, wherein the cellulose oligomer is represented by the following general formula (1):
- wherein A represents an alkyl group having 1 to 12 carbon atom(s), and n represents an average polymerization degree of 5 to 20, wherein said cellulose oligomer is present in an amount sufficient to retain said cells in a suspended state during said suspension culturing.
Dependencies are inferred from the claim text.