Compositions comprising cell-delivered vesicles and uses thereof
AssigneeProKidney
InventorsTimothy A. Bertram, Deepak Jain
Life of this patent
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Abstract
Provided herein are, inter alia, extracellular products (e.g., vesicles such as microvesicles, e.g., exosomes) produced by renal cells (such as bioactive renal cells, e.g., selected renal cells). Methods of altering components (such as miRNAs or proteins) of vesicles produced by cells, as well as methods of producing vesicles comprising various compounds are also included. Also provided are diagnostic and treatment methods.
Claims23
A method for detecting whether a vesicle secreted by a renal cell is regenerative and for treatment of a renal disease in a subject with a vesicle preparation comprising the vesicle having been identified as regenerative, the method comprising:
- (i) obtaining the vesicle secreted by the renal cell;
- (ii) detecting whether at least one protein is upregulated in the vesicle compared to vesicles secreted by unpassaged primary renal cells,
- wherein the at least one protein is CD9, CD326, CD40, and/or CD49e;
- (iii) identifying the vesicle as regenerative if the at least one protein is upregulated in the vesicle;
- and
- (iv) administering to a subject in need of treatment for renal disease a vesicle preparation comprising an effective amount of the vesicle having been identified in (iii) as regenerative.
A method for detecting whether a vesicle secreted by a renal cell is regenerative and for treatment of a renal disease in a subject with a vesicle preparation comprising the vesicle having been identified as regenerative, the method comprising:
- (i) obtaining the vesicle secreted by the renal cell;
- (ii) detecting whether at least one protein is downregulated in the vesicle compared to vesicles secreted by unpassaged primary renal cells,
- wherein the at least one protein is CD42a, and/or SSEA-4;
- (iii) identifying the vesicle as regenerative if the at least one protein is downregulated in the vesicle;
- and
- (iv) administering to a subject in need of treatment for renal disease a vesicle preparation comprising an effective amount of the vesicle having been identified in (iii) as regenerative.
Dependencies are inferred from the claim text.