Means to detect whether acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus is virulent or non-virulent
AssigneeUniversiteit Gent
InventorsPeter Bossier, Vikash Kumar, Suvra Roy
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Abstract
A method of monitoring acute hepatopancreatic necrosis disease in shrimp is disclosed. More specifically, detection of the presence of alkaline phosphatase Phox enzyme, or, of the PiRAVP and/or the PirBVP toxins of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus correlates with non-virulence or virulence, respectively, of the bacterium is disclosed. Hence, an assay capable of detecting the Phox enzyme and/or the toxins could be very useful to monitor the disease in shrimp.
Claims7
A method of phenotyping Vibrio parahaemolyticus as non-virulent, the method comprising:
- providing a sample comprising shrimp culture water;
- utilizing antibodies or fragments thereof that bind to alkaline phosphatase Phox enzyme of V. parahaemolyticus to phenotype the V. parahaemolyticus as non-virulent;
- and
- culturing the shrimp without disease-mitigation treatment if the V. parahaemolyticus are phenotyped as non-virulent.
An assay comprising:
- antibodies or fragments thereof that bind to the alkaline phosphatase Phox enzyme of Vibrio parahaemolyticus.
A process of phenotyping Vibrio parahaemolyticus as non-virulent, the process comprising:
- providing a sample comprising shrimp culture water;
- bringing the sample into contact with antibodies or fragments thereof that bind to alkaline phosphatase Phox enzyme of V. parahaemolyticus;
- determining whether the alkaline phosphatase Phox enzyme of V. parahaemolyticus is present in the sample;
- classifying the V. parahaemolyticus s non-virulent when the alkaline phosphatase Phox enzyme is present in the sample;
- and
- culturing the shrimp without disease-mitigation treatment upon classification of the V. parahaemolyticus as non-virulent.
Dependencies are inferred from the claim text.