Dual stationary phase gradient columns for new selectivities in liquid chromatography
AssigneeWaters Technologies Corporation
InventorsSzabolcs Fekete, Matthew A. Lauber
Life of this patent
Estimating the term…
Abstract
The present disclosure is directed to liquid chromatography columns and methods utilizing a stationary phase sorbent having two or more gradient factors. Each of the two or more gradient factors varies in a progressive manner along a length of the stationary phase sorbent in a direction from an inlet to an outlet of the column (i.e., along a length of the column). As a result of including these new continuous stationary phase gradients, new selectivities allowing for separation and analysis of complex samples including large biomolecules is achievable.
Claims20
A chromatographic column comprising:
- a housing with an inlet and an outlet;
- and
- a stationary phase sorbent contained within the housing and extending from the inlet to the outlet, wherein the stationary phase sorbent is defined by two or more retention gradients that vary in a progressive manner along a length of the stationary phase sorbent in a direction from the inlet to the outlet of the housing.
A method of improving a range of selectivity of a chromatographic separation, the method including:
- providing a chromatographic column, wherein the column includes a stationary phase sorbent material having two gradients that vary progressively along a length of the column;
- and
- applying a mobile phase gradient to separate a sample injected into the chromatographic column.
A method of modifying selectively for a chromatographic separation, the method comprising:
- controlling packing of a chromatographic column with a two phase stationary phase sorbent, the two phase stationary phase sorbent comprising a first sorbent material and a second sorbent material, the first sorbent material differing from the second sorbent material, wherein the first sorbent material is disposed within the chromatographic column to provide a first gradient factor of greater than 1 from an inlet to an outlet and the second sorbent material is disposed within the chromatographic column to provide a second gradient factor of greater than 1 from an inlet to the outlet.
Dependencies are inferred from the claim text.