US 12,442,768 B2Granted

Photostimulation device and methods of using the same

AssigneesThe Trustees of the University of Pennsylvania, The Trustees of Princeton University

InventorsWilliam Benman, Lukasz Bugaj, Brian Chow, Saachi Datta, David Gonzalez-Martinez, Jose Avalos

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Abstract

Provided herein is a stimulation and measurement device. The stimulation and measurement device includes a microwell plate having a top side, a bottom side, and at least one well extending from the top side towards the bottom side; a first assembly removably positioned on the top side of the microwell plate; a second assembly removably positioned on the bottom side of the microwell plate; and a microcontroller. The first assembly includes a first printed circuit board having at least one pair of stimulating light emitting diodes (LEDs) positioned thereon to align with one of the wells. The second assembly includes a second printed circuit board having at least one photodiode positioned thereon to align with one of the pairs of stimulating LEDs, and at least one excitation LED paired with each of the photodiodes. The microcontroller is configured to control the stimulating LED(s), the photodiode(s), and the excitation LED(s).

Claims20

Dependent claims

A stimulation and measurement device comprising:

a microwell plate comprising:
a top side;
a bottom side;
and
at least one well extending from the top side towards the bottom side;
a first assembly removably positioned on the top side of the microwell plate, the first assembly comprising:
a first printed circuit board;
and
at least one pair of stimulating light emitting diodes (LEDs), each of the at least one pair of stimulating LEDs arranged and disposed on the first printed circuit board to align with one of the at least one wells;
a second assembly removably positioned on the bottom side of the microwell plate, the second assembly comprising:
a second printed circuit board;
at least one photodiode, each of the at least one photodiodes arranged and disposed on the second printed circuit board to align with one of the at least one pairs of stimulating LEDs;
and
at least one excitation LED paired with each of the at least one photodiodes;
and
a microcontroller configured to control the at least one pair of stimulating LEDs, the at least one photodiode, and the at least one excitation LED.

13 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/467,469Filed 14 Sep 2023 · us provisional application
Priority: 14 Sep 2022
Published: US 2024/0085332 A114 Mar 2024
Granted: 14 Oct 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 21/6452
Specially adapted constructive features of fluorimeters: individual samples arranged in a regular 2D-array, e.g. multiwell plates
G01N 21/6486
Fluorescence; Phosphorescence: measuring fluorescence of biological material, e.g. DNA, RNA, cells
G01N 21/253
Colorimeters; Construction thereof: for batch operation, i.e. multisample apparatus
G01N 21/6408
Fluorescence; Phosphorescence: with measurement of decay time, time resolved fluorescence