Bond-selective intensity diffraction tomography and uses thereof
AssigneeTrustees of Boston University
InventorsJi-Xin Cheng, Jian Zhao, Lei Tian, Alex Matlock
Life of this patent
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Abstract
An example microscope includes a pump laser for providing a first illumination to a sample. A laser array provides a second illumination to the sample. The laser array may include a plurality of laser elements, each providing oblique illuminations to the sample. An illumination collecting source collects the first illumination and the second illumination from the sample. The illumination collecting source may capture transient 3D refractive index (RI) variations in the sample due to the first illumination and second illumination.
Claims22
An imaging system comprising:
- an illumination source for providing oblique illuminations to a sample, the illumination source including a plurality of laser elements and a pump laser that are synchronized to produce the oblique illuminations, wherein the plurality of laser elements is arranged to form a ring of laser elements such that each laser element on the ring of laser elements is positioned to produce an oblique illumination on the sample;
- and
- a camera for collecting scattered illuminations from the sample, the camera producing intensity images encoding a volume of the sample for each of the oblique illuminations, wherein the camera captures three-dimensional chemical-specific refractive index (RI) variations of the sample due to the oblique illuminations using the intensity images.
A method for microscopic imaging comprising:
- providing, using an illumination source, oblique illuminations to a sample, the illumination source including a plurality of laser elements and a pump laser that are synchronized to produce the oblique illuminations, wherein the plurality of laser elements is arranged to form a ring of laser elements such that each laser element on the ring of laser elements is positioned to produce an oblique illumination on the sample;
- and
- using a camera, collecting scattered illuminations from the sample and producing intensity images encoding a volume of the sample for each of the oblique illuminations, wherein the camera captures three-dimensional chemical-specific refractive index (RI) variations of the sample due to the oblique illuminations using the intensity images.
Dependencies are inferred from the claim text.