Flow cell
AssigneeCytonome/St, LLC
InventorsJohnathan Charles Sharpe, Rudolf Hulspas, Kristopher Scott Buchanan
Life of this patent
Estimating the term…
Abstract
A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region.
Claims16
A droplet sorting method comprising:
- forming a hydrodynamically focused stream of particles by flowing a stream including particles through at least one hydrodynamic focusing region provided in an operatively sealed sort chamber;
- introducing the hydrodynamically focused stream of particles through a jet-forming element into an enclosed region provided in the sort chamber;
- applying excitation light from an excitation source to the hydrodynamically focused stream of particles downstream of the jet-forming element;
- transforming the hydrodynamically focused stream of particles into a series of droplets;
- and
- deflecting a droplet from the series of droplets in a droplet deflection region based on a charge applied to the droplet.
Dependencies are inferred from the claim text.