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US 12,442,030 B2Granted
Methods and systems for determining ADAMTS13 enzyme activity
AssigneeLaboratory Corporation of America Holdings
InventorsRussell Philip Grant, Christopher Michael Shuford, Meghan Norris Bradley
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Abstract
Disclosed are methods and systems for the analysis activity of enzyme disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) in a sample. The methods and systems disclosed herein can be useful for diagnosis of thrombotic thrombocytopenic purpura in a patient.
Claims19
A system for determining activity of enzyme disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) in a sample, the system comprising:
- a station for incubating the sample with an exogenous peptide substrate for ADAMTS13 under conditions allowing for enzymatic cleavage of the exogenous peptide substrate by ADAMTS13 to generate an enzymatic cleavage product, wherein the exogenous peptide substrate consists of an amino acid sequence with at least 90% sequence identity to SEQ ID NO:
- 3 and comprises an ADAMTS13 cleavage site;
- a station for ionizing the enzymatic cleavage product to generate a multiply charged gas-phase ion of the enzymatic cleavage product;
- and,
- a station for analyzing the multiply charged gas-phase ion by tandem mass spectrometry to determine presence and/or amount of the enzymatic cleavage product in the sample, wherein the presence or the amount of the enzymatic cleavage product is indicative of the activity of ADAMTS13 in the sample.
2 more elements
Dependencies are inferred from the claim text.