Production of fatty acyl-CoA in yeast using a fatty acid feedstock
AssigneePyrone Systems, Inc.
InventorsAlexander Hutagalung, Jose Miguel Laplaza
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Abstract
Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.
Claims19
A method of producing a fatty acyl-CoA in a peroxisome, the method comprising:
- providing a fatty acid to a genetically modified microorganism, wherein the genetically modified organism is a yeast or a fungus, wherein the microorganism comprises a biosynthetic enzyme targeted to the peroxisome, wherein the biosynthetic enzyme is capable of catabolizing the fatty acid into the fatty acyl-CoA;
- culturing the microorganism under conditions sufficient to produce the fatty acyl-CoA in the peroxisome;
- and
- isolating the fatty acyl-CoA.
A genetically modified microorganism, comprising a biosynthetic enzyme targeted to a peroxisome in the microorganism, wherein the genetically modified microorganism is a yeast or a fungus, wherein the biosynthetic enzyme is capable of catabolizing a fatty acid into a fatty acyl-CoA.
Dependencies are inferred from the claim text.