Engineered polypeptides and its application in the synthesis of tyrosine or tyrosine derivatives
AssigneeEnzymaster (Ningbo) Bio-Engineering Co., Ltd
InventorsHaibin Chen, Chengxiao Zhang, Shumin Shi, Baoqin Cai, Lei Sun, Qinli Peng, Zikun Wang, Yong Koy Bong
Life of this patent
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Abstract
Provided are efficient catalyst of engineered enzymes and an economical enzymatic reaction solution to solve the problems in the current production process of L-tyrosine and its derivatives. The method of the invention has the advantages of high product concentration, mild reaction conditions, simple purification process, simple operation, environmental friendliness, and easy industrial scale-up. Thus, it has good industrial application prospects.
Claims22
An engineered polypeptide that catalyzes the synthesis of L-tyrosine in enantiomeric excess, said polypeptide comprising an amino acid sequence having at least one amino acid substitution relative to SEQ ID NO:
- 2, wherein said at least one amino acid substitution is selected from the group consisting of X132R, X354P, X454E, and combinations thereof, further wherein said amino acid sequence is selected from the group consisting of SEQ ID No:
- 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, and 308.
An engineered polypeptide, which is a polypeptide of (a) or (b) below:
- (a) a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID No:
- 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, and 308;
- or
- (b) a polypeptide having tyrosine phenol-lyase activity, which comprises an amino acid sequence having (i) at least 95% sequence identity to one of the amino acid sequences recited in (a), and (ii) more of X132R, X354P, and X454E amino acid substitutions relative to said one amino acid sequence recited in (a).
Dependencies are inferred from the claim text.