Enzyme nanoparticles with methane oxidation activity
AssigneeKorea University Research and Business Foundation
InventorsJeewon Lee, Hyun Jin Kim
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Abstract
The present invention relates to novel enzyme nanoparticles capable of converting methane into methanol, in which key active sites of methane-oxidizing bacteria are fused with each other and expressed on a protein that can be self-assembled in cells to form nanoparticles, and specifically to enzyme nanoparticles including a protein having methane monooxygenase (MMO) activity and active sites of the methane oxidase, a method for production thereof, a recombinant microorganism into which a nucleic acid encoding the protein and the active site of the methane oxidase is introduced, and immobilized enzyme nanoparticles including the enzyme nanoparticles loaded on a carrier.
Claims3
An enzyme nanoparticle comprising:
- a first active domain of a methane monooxygenase (MMO) comprising the amino acid sequence of SEQ ID NO:
- 2 and a second active domain of the MMO comprising the amino acid sequence of SEQ ID NO:
- 3;
- and a human ferritin heavy-chain protein, wherein the first active domain and the second active domain are fused to a surface of the human ferritin heavy-chain protein, wherein the enzyme nanoparticle converts methane to methanol, and wherein the MMO and the human ferritin heavy-chain protein are linked by a peptide linker.
An enzyme nanoparticle comprising:
- a first active domain of a methane monooxygenase (MMO) comprising the amino acid sequence of SEQ ID NO:
- 4 and a second active domain of the MMO comprising the amino acid sequence of SEQ ID NO:
- 3;
- and a human ferritin heavy-chain protein, wherein the first active domain and the second active domain are fused to a surface of the human ferritin heavy-chain protein, wherein the enzyme nanoparticle converts methane to methanol, and wherein the MMO and the human ferritin heavy-chain protein are linked by a peptide linker.
An enzyme nanoparticle comprising:
- a first active domain of a methane monooxygenase (MMO) comprising the amino acid sequence of SEQ ID NO:
- 5 and a second active domain of the MMO comprising the amino acid sequence of SEQ ID NO:
- 3;
- and a human ferritin heavy-chain protein, wherein the first active domain and the second active domain are fused to a surface of the human ferritin heavy-chain protein, wherein the enzyme nanoparticle converts methane to methanol, and wherein the MMO and the human ferritin heavy-chain protein are linked by a peptide linker.
Dependencies are inferred from the claim text.