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US 12,442,007 B2Granted
Method for producing target protein
AssigneeNagase Viita Co., Ltd.
InventorKoichi Harazono
Life of this patent
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Abstract
Provided is a method which can produce a target protein while stably maintaining a vector without any special genetic manipulation of host cells and without use of a drug resistance gene or the like. A method for producing a target protein including culturing cells transformed with a vector, the vector containing a gene of the target protein and not containing an antibiotic resistance gene, a recombinase recognition sequence, or a gene essential for cell survival.
Claims5
A method for producing a target protein, comprising culturing cells transformed with a vector,
- wherein the vector comprises a gene of the target protein and does not comprise an antibiotic resistance gene, a recombinase recognition sequence, nor a gene essential for cell survival,
- wherein the target protein is phospholipase, glucanase, or protease, and
- wherein the vector further comprises a replication initiator sequence obtained from pIJ702 or pIJ350.
Dependencies are inferred from the claim text.