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US 12,440,581 B2Granted
Fluorophores for super-resolution imaging
AssigneeHoward Hughes Medical Institute
InventorsLuke D. Lavis, Qinsi Zheng
Life of this patent
Estimating the term…
Abstract
The presently-disclosed subject matter includes fluorescent compounds of the following formula: The compounds can be used as probes, dyes, tags, and the like. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.
Claims24
A compound of the formula:
- or a closed form thereof, wherein
- (a) X is selected from the group consisting of O, N-alkyl, S, and C(alkyl)2;
- Y is —NH2 or
- wherein each Y1 and Y2 is each independently selected from the group consisting of H, F, CN, OCH3, SO2CH3, CF3, CH3, and CO2H;
- R1 and R2 are each independently selected from the group consisting of H, alkyl, and halogen;
- R3, which can be a substitution at either the 5 position or the 6 position of the ring to which it is bound, is selected from the group consisting of H, self-labeling protein tag ligand, CO2H, CO2CH3, CO2t-Bu, N-hydroxysuccinimidyl (NHS) ester, and a targeting moiety;
- and
- R4 is CH2OH;
- (b) X is Si(alkyl)2;
- Y is —NH2;
- R1 and R2 are each independently selected from the group consisting of H, alkyl, and halogen;
- R3, which can be a substitution at either the 5 position or the 6 position of the ring to which it is bound, is selected from the group consisting of H, self-labeling protein tag ligand, CO2H, CO2CH3, CO2t-Bu, NHS ester, and a targeting moiety;
- and
- R4 is CH2OH;
- (c) X is Si(alkyl)2;
- Y is
- wherein each Y1 and Y2 is each independently selected from the group consisting of F, CN, OCH3, SO2CH3, CF3, CH3, and CO2H;
- R1 and R2 are each independently selected from the group consisting of H, alkyl, and halogen;
- R3, which can be a substitution at either the 5 position or the 6 position of the ring to which it is bound, is selected from the group consisting of H, self-labeling protein tag ligand, CO2H, CO2CH3, CO2t-Bu, NHS ester, and a targeting moiety;
- and
- R4 is CH2OH;
- (d) X is Si(alkyl)2;
- Y is
- wherein Y1 and Y2 are H;
- R1 and R2 are each independently selected from the group consisting of alkyl, and halogen;
- R3, which can be a substitution at either the 5 position or the 6 position of the ring to which it is bound, is selected from the group consisting of H, self-labeling protein tag ligand, CO2H, CO2CH3, CO2t-Bu, NHS ester, and a targeting moiety;
- and
- R4 is CH2OH;
- or
- (e) X is Si(alkyl)2;
- Y is
- wherein Y1 and Y2 are H;
- R1 and R2 are H;
- R3, which can be a substitution at either the 5 position or the 6 position of the ring to which it is bound, is selected from the group consisting of H, self-labeling protein tag ligand, CO2H, CO2CH3, CO2t-Bu, and a targeting moiety;
- and
- R4 is CH2OH.
33 more elements
Dependencies are inferred from the claim text.