Genetically modified cell line for production of Marek's disease virus vaccine and methods of making and using the same
AssigneeZoetis Services LLC
InventorsKeith Allen Ameiss, Everett Lee Rosey
Life of this patent
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Abstract
The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.
Claims23
A genetically modified avian cell line capable of supporting high-titer growth of a Marek's Disease Virus (MDV), wherein the genetic modification comprises a genetic alteration of one or more of the following genes:
- SLAMF8, HTR2A, STAT4, CTSL2, and COBLL1;
- and wherein high-titer growth refers to at least a 2-fold higher yield in the viral titer as that achieved with the same MDV strain in a parent avian cell line.
A method of producing a genetically modified cell line capable of supporting high-titer growth of Marek's Disease Virus (MDV), the method comprising:
- providing a starting cell line, wherein said starting cell line is an immortalized avian cell line, and producing a genetically modified cell line by genetically altering the starting cell line to reduce expression thereby altering functional activity of the product of one or both of gene HTR2A and SLAMF8, wherein the genetically modified cell line is capable of supporting increased MDV viral titer as compared to the starting cell line when infected with the same MDV strain.
Dependencies are inferred from the claim text.