System and a method for imaging using lens-less holographic microscopy

A method of imaging, comprising:

• illuminating a sample comprising an object via a stationary illumination source positioned at an object-source distance z2 from the sample on a first side of the sample;
• capturing a lens-less holographic image of the sample illuminated with light originating from the stationary illumination source via a stationary image sensor positioned at an object-sensor distance z1 from the sample on a second side opposite the first side of the sample;
• and
• performing wavefront reconstruction of the lens-less holographic image of the sample to produce three-dimensional details of the object,
• wherein the stationary illumination source comprises a coherent light source configured to produce a divergent coherent light, wherein the divergent coherent light is scattered by the object of interest in the sample to produce scattered light that interferes with the divergent coherent light to produce interference patterns,
• wherein the lens-less holographic image of the sample captured by the stationary image sensor comprises the interference patterns, and
• wherein performing the wavefront reconstruction of the lens-less holographic image of the sample comprises applying equations governing coherent light propagation for both spherical waves and planar waves in a reconstruction of the interference patterns in producing the three-dimensional details the object.

A holographic imaging device, comprising:

• a stationary illumination source configured to illuminate a sample comprising an object, the stationary illumination source positioned at an object-source distance z2 from the sample on a first side of the sample;
• and
• a stationary imaging module configured to capture a lens-less holographic image of the sample illuminated with light originating from the stationary illumination source, the stationary imaging module being located at an object-sensor distance z1 from the sample on a second side opposite the first side of the sample,
• wherein:
• the stationary imaging module is further configured to perform wavefront reconstruction of the lens-less holographic image of the sample to produce three-dimensional details of the object,
• the stationary illumination source comprises a coherent light source configured to produce a divergent coherent light,
• the divergent coherent light is scattered by the object of interest in the sample to produce scattered light that interferes with the divergent coherent light to produce interference patterns,
• the lens-less holographic image of the sample captured by the stationary imaging module comprises the interference patterns, and
• the stationary imaging module is further configured to apply equations governing coherent light propagation for both spherical waves and planar waves in a reconstruction of the interference patterns to produce the three-dimensional details of the object.