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US 12,436,156 B2Granted
Methods of determining pH and calcium or chloride concentration in samples
AssigneeThe University of Chicago
InventorsYamuna Krishnan, Kasturi Chakraborty, Ka Ho Leung, Anand Saminathan
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Abstract
This disclosure relates to methods for determining pH and also calcium (Ca2+) concentration or chloride (Cl−) concentration in biological samples. More particularly, this disclosure relates to methods capable of simultaneously determining pH and Ca2+ concentration, or pH and Cl− concentration using nucleic acid complexes.
Claims18
A method for simultaneously determining 1) pH, and 2) Ca2+ concentration or Cl− concentration in a sample comprising:
- providing a nucleic acid complex comprising
- a first single-stranded nucleic acid molecule comprising a Ca2+ fluorophore or a Cl− fluorophore crosslinked to the first strand;
- and
- a second single-stranded nucleic acid molecule that is partially or fully complementary to the first single-stranded molecule,
- wherein the nucleic acid complex further comprises a first label conjugated to the first single-stranded nucleic acid molecule or the second single-stranded nucleic acid molecule and the first label is capable of producing a signal, wherein the intensity of the signal is dependent on change in pH;
- measuring the intensity of the signal;
- and
- determining the pH, and Ca2+ or Cl− concentration from the measured signal by comparing to a reference value.
5 more elements
Dependencies are inferred from the claim text.