Detection of antibodies against RAN proteins from serum and tissue lysates

A method comprising:

• (i) detecting in a first biological sample obtained from a subject one or more anti-RAN protein antibodies using an electrochemiluminescence-based immunoassay;
• (ii) detecting in a second biological sample obtained from the subject one or more anti-RAN protein antibodies using an electrochemiluminescence-based immunoassay, wherein the second biological sample is obtained after administration of a therapeutic agent to the subject;
• (iii) comparing an amount of anti-RAN protein antibodies detected in the second biological sample to an amount of anti-RAN protein antibodies detected in the first biological sample;
• (iv) identifying the subject as having a change in one or more anti-RAN protein antibody levels when the amount of anti-RAN protein antibodies detected in the second biological sample is different than the amount of anti-RAN protein antibodies detected in the first biological sample;
• and
• (v) administering to the subject a vaccine against a RAN protein-associated disease or disorder when the second biological sample contains an increased level of anti-RAN protein antibodies, relative to the first biological sample.