Methods, compositions, and kits for high-throughput release, labeling, and analysis of O-glycans present on a glycoconjugate
AssigneeAgilent Technologies, Inc.
InventorAce Gita Galermo
Life of this patent
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Abstract
The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.
Claims20
A method for releasing, labeling, and, optionally, analyzing, O-glycans present on a selected glycoconjugate of interest, said method comprising:
- incubating in a container said selected glycoconjugate of interest in an aqueous solution comprising 1.20M-2M triethylamine (“TEA”), and 0.20M to 1.25M 1-phenyl-3-methyl-5-pyrazolone (“PMP”) at a temperature of 70-100° C. for a time between about 10 minutes and 23 hours.
A composition comprising a selected glycoconjugate of interest and an aqueous solution comprising 1.20M-2M triethylamine (“TEA”), and 0.20M to 1.25M 1-phenyl-3-methyl-5-pyrazolone (“PMP”).
A kit for releasing, labeling, and, optionally, analyzing, O-glycans present on a selected glycoconjugate of interest, said kit comprising:
- (a) a container, (b) triethylamine (“TEA”), and (c) 1-phenyl-3-methyl-5-pyrazolone (“PMP”).
Dependencies are inferred from the claim text.