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US 12,435,452 B2Granted
Enzyme extraction methods
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsEdward Kavazanjian, Nasser Hamdan, Hamed Khodadadi Tirkolaei, Neda Javadi
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Abstract
Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.
Claims4
A method of producing a powdered crude extract for biocementation, the method consisting of:
- soaking a seed in an aqueous solution for between 12 and 24 hours to form a soaked seed, wherein the seed comprises jack bean, and wherein the aqueous solution consists of water;
- subsequently homogenizing the soaked seed to produce a homogenized suspension with urease proteins and non-urease proteins;
- filtering the homogenized suspension to produce a crude extract, wherein a purification fold of the crude extract is equal to 1;
- and
- lyophilizing and/or drying the crude extract to produce the powdered crude extract, and wherein:
- the powdered crude extract comprises urease proteins and non-urease proteins, and
- the powdered crude extract is produced for a biocementation process for enzyme induced carbonate precipitation (EICP) treatment of soil.
4 more elements
Dependencies are inferred from the claim text.