US 12,435,376 B2 · Method for high-sensitivity and high-specificity detection of biomolecules by using mass spectrometry

Abstract

The present invention relates to a method for high-sensitivity and high-specificity detection of biomolecules by using mass spectrometry and, more specifically, to a method for high-sensitivity and high-specificity detection of proteins such as miRNA or antigens by using time-of-flight secondary ion mass spectrometry (ToF-SIMS), matrix-assisted laser desorption/ionization (MALDI), or laser desorption/ionization (LDI) mass spectrometry. The method for high-sensitivity and high-specificity detection of biomolecules by using mass spectrometry, according to the present invention, enables high-sensitivity and high-specificity detection of biomolecules by using surface mass spectrometry, and the method is expected to be used for the diagnosis and prediction of diseases by quantifying, from a biosample, a target probe such as miRNA which is known as a disease marker.

Claims11

Dependent claims

A method for quantification of biomolecules by using mass spectrometry, the method comprising:

generating a precipitate to quantify the biomolecules by:
i) fixing a first probe molecule in a metal;
ii) reacting the biomolecules with the fixed first probe molecule to form a biomolecule complex;
iii) reacting a second probe molecule with the biomolecule complex, which is formed by reacting the biomolecules with the fixed first probe molecule, so that the second probe molecule binds to the biomolecule complex;
and
iv) binding an enzyme conjugate to the bound second probe molecule, wherein the enzyme conjugate is a conjugate in which a material interacting with the second probe molecule is conjugated with the enzyme;
reacting a substrate with the enzyme of the enzyme conjugate to form an insoluble product;
and
quantifying the insoluble product by using mass spectrometry,
wherein the biomolecules are microRNA (miRNA).

7 more elements

Dependencies are inferred from the claim text.

Method for high-sensitivity and high-specificity detection of biomolecules by using mass spectrometry

Life of this patent

Method for high-sensitivity and high-specificity detection of biomolecules by using mass spectrometry

Life of this patent

Abstract

Claims11