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US 12,435,340 B2Granted
Methods for transformation of dicot plant cells
AssigneeSyngenta Crop Protection AG
InventorsJianping Xu, Chao Li, Qin Wen
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Abstract
This disclosure relates to methods of transformation of dicots, such as tomato and watermelon. In some aspects, the methods comprise use of dicot explants obtained by dividing seeds and soaking the dicot explants in various media. In some aspects, transformed cells of a dicot plant are provided as well as dicot plants comprising transformed cells.
Claims16
A method of transforming a tomato plant cell, comprising:
- (a) obtaining a tomato plant seed;
- (b) soaking the tomato plant seed in a seed-soak medium;
- (c) dividing the soaked tomato plant seed of step (b) into at least 10 tomato explants;
- (d) inoculating the at least 10 tomato explants of step (c) with Agrobacterium comprising a nucleic acid of interest, thereby obtaining Agrobacterium-inoculated tomato explants;
- and
- (e) co-cultivating the Agrobacterium-inoculated tomato explants to produce a transformed tomato plant cell, wherein the soaking step of step (b) is within the range of 1 hour to 72 hours, with or without light, wherein the method produces less than 15% aberrant tetraploid transformants, wherein the plurality of tomato explants is at least 10 discrete explants.
3 more elements
Dependencies are inferred from the claim text.