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US 12,435,326 B2Granted
Lactase enzymes with improved properties
AssigneeKerry Group Services International Limited
InventorsHans Raj, Pernille Smith, Thomas Eckhardt, Vojislav Vojinovic, Charlotte Elisabeth Grüner Schöller, Johannes Maarten Van Den Brink
Life of this patent
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Abstract
The present invention relates to new improved peptides and dimeric peptides exhibiting beta-galactosidase enzyme activity as well as methods using them for reducing the lactose content of milk-based compositions.
Claims12
A method for reducing lactose content of a milk-based substrate, comprising:
- (a) adding a peptide to a milk-based substrate having a lactose concentration of at least 10 g/L to obtain a mixture comprising the milk-based substrate and the peptide, wherein the peptide comprises an amino acid sequence at least 95% identical to the amino acid sequence of G83 (SEQ ID NO:12), G95 (SEQ ID NO:14), G100 (SEQ ID NO:15), G108 (SEQ ID NO:17), or G109 (SEQ ID NO:18), and
- (b) incubating the mixture at a temperature of from 1° C.-10° C., wherein the peptide is added to the milk-based substrate at a concentration of 10 to 55 mg/L of the milk-based substrate;
- wherein the peptide exhibits beta-galactosidase enzyme activity at the incubating temperature and reduces the lactose concentration in the mixture to less than 0.2% after incubation for 4-8 hours.
Dependencies are inferred from the claim text.