Generation of CTL lines with specificity against multiple tumor antigens or multiple viruses
AssigneesBaylor College of Medicine, Wilson Wolf Manufacturing
InventorsAnn Marie Leen, Ulrike Gerdemann, Cliona M. Rooney, Juan F. Vera Valdes, John R. Wilson
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Abstract
The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and/or that employ a particular bioreactor having a gas permeable membrane.
Claims8
A method of producing a cultured polyclonal population of cytotoxic T-lymphocytes (CTLs) that recognize at least one epitope from each of at least two different tumor antigens, said population comprising CD4+ T-lymphocytes and CD8+ T-lymphocytes, comprising the steps of:
- ia) stimulating DCs or PBMCs with at least two libraries of peptides that each represent at least one epitope from each of at least two different tumor antigens to generate antigen presenting cells (APCs) and stimulating T cells with the APCs to generate antigen-specific T lymphocytes;
- or
- ib) stimulating PBMCs with at least two libraries of peptides that each represent at least one epitope from each of at least two different tumor antigens to generate antigen-specific T-lymphocytes;
- and
- ii) culturing the antigen-specific T-lymphocytes in the presence of IL-7 and IL-15 to generate a population of CTLs.
Dependencies are inferred from the claim text.