Method and apparatus for high-throughput maskless fabrication of polymer scaffolds and biological tissues in multi-well plates

A high-throughput, maskless fabrication apparatus for fabricating scaffolds or constructs, comprising:

• a light source to produce a probe light;
• a digital display device to receive the probe light and to cause spatial modulation of the received probe light to produce a programmed spatial pattern in a spatially-modulated light;
• a stage to hold a target surface or chamber including a multi-well plate comprising two or more wells, wherein the target surface or chamber contains at least one solution including a material or multiple materials that forms a scaffold or construct based on interaction with the spatially-modulated light projected at the solution;
• and
• a computer control device in communication with the light source and the digital display device to control a change of the solution including the material or multiple materials to form the scaffold or construct,
• wherein the digital display device is configured to generate optical patterns which are projected from beneath the multi-well plate to (i) an entire bottom of the multi-well plate, thereby exposing the solution contained in two or more different wells to the optical patterns such that selective photopolymerization of the solution occurs in each of the two or more different wells simultaneously or (ii) a part of a bottom of the multi-well plate, thereby exposing the solution contained in two or more different wells to the optical patterns such that selective photopolymerization of the solution occurs in each of the two or more different wells simultaneously.