Fc-epsilon CAR
AssigneeImmunityBio, Inc.
InventorsLaurent H. Boissel, Hans G. Klingemann, Abhijit Dandapat, Himani Chinnapen
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Abstract
Recombinant NK cells, and especially recombinant NK-92 cells express a chimeric antigen receptor (CAR) having an intracellular domain of FcεRIγ. Notably, CAR constructs with an intracellular domain of FcεRIγ had a substantially prolonged duration of expression and significantly extended cytotoxicity over time. The CAR may be expressed from RNA and DNA, preferably as a tricistronic construct that further encodes CD16 and a cytokine to confer autocrine growth support. Advantageously, such constructs also enable high levels of transfection and expression of the recombinant proteins and provide a convenient selection marker to facilitate rapid production of recombinant NK/NK-92 cells.
Claims8
A genetically modified NK cell, comprising:
- a cytokine;
- a CD16;
- and
- a membrane bound chimeric antigen receptor (CAR) that comprises a FcεRIγ signaling domain having the amino acid sequence of SEQ ID NO:1;
- and
- wherein the CAR is
- a CD19-CAR having the amino acids 1-407 of SEQ ID NO:29,
- a PD-L1-CAR encoded by a nucleic acid sequence of SEQ ID NO:42, or
- a B7-H4-CAR encoded by a nucleic acid sequence of SEQ ID NO:45.
Dependencies are inferred from the claim text.