Rapid individualized whole blood chip for antibiotic, drug, and food allergies

A microfluidic chip comprising:

• (a) an incubation chamber comprising
• an incubation inlet,
• an incubation outlet connected to a wash inlet, and a vertical fluidic barrier, wherein said fluidic barrier blocks the diffusion of particles in a solution of 0.1-20 microns in size;
• (b) a wash chamber comprising the wash inlet connected to the incubation outlet and a wash outlet connected to a readout inlet;
• and
• (c) a readout chamber comprising the readout inlet, a readout channel, and a readout outlet connected to the wash outlet and a readout outlet connected to a readout channel, wherein said readout channel comprises a series of sequentially ordered seats having a concave shape with an opening and a separation between the base of the seats, and
• wherein said readout chamber is configured to allow for a dual flow of the solution through the readout chamber, wherein said dual flow comprises:
• an unblocked main flow of the solution from the readout inlet to the readout outlet;
• and
• a shunt flow of the solution through the opening and separation at the base of the seats, and
• wherein the shunt flow of the solution through the readout chamber exhibits a hydrodynamic resistance ratio between the hydrodynamic resistance of the shunt flow direction through the first empty seat (R1) and the hydrodynamic resistance of the shunt flow direction through the next empty seat (R2) is from 1 to 3, wherein R1 is smaller than R2, wherein the hydrodynamic resistance is calculated using the solution for Poiseuille flow in a rectangular channel.