Microfluidic sample preparation device offering high repeatability
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Horiba Abx SAS
InventorsYves Fouillet, Anais Ali-Cherif
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Abstract
A device for preparing a solution from a sample and a reagent, the device includes a microfluidic array having a sample supply inlet, a reagent supply inlet, a discharge outlet, a solution collection outlet, a sampling zone to which the inlets are connected, first and second preparation chambers connected to the sampling zone, arranged to either side of the sampling zone such that the liquid flowing from one preparation chamber to the other flows through the first sampling zone, the first preparation chamber having a volume that is variable between a minimum volume and a calibrated volume. The device includes valves interrupting the flow of the fluid at least at the two inlets and the collection and discharge outlets.
Claims14
A device for preparing at least one solution having a calibrated volume Vc from at least one first sample and at least one first reagent, comprising:
- a microfluidic network including at least one first supply inlet for a first sample, at least one second supply inlet for a first reagent, at least one discharge outlet, at least one collection outlet for the at least one solution to be analysed, a first sampling zone having a calibrated volume Ve to which the first inlet and second inlet are directly connected, at least first preparation chamber and second preparation chamber connected to the first sampling zone and disposed on either side of the first sampling zone so that the fluid for circulating from one preparation chamber to the other circulates in the first sampling zone,
- wherein the at least one first supply inlet is fluidly connected to the first sampling zone on a first side of the first sampling zone, and the at least one second supply inlet is fluidly connected to the first sampling zone on a second side of the sampling zone opposite to the first side,
- wherein the first preparation chamber has a variable volume between a minimum volume V0 and the calibrated volume Vc,
- wherein the second preparation chamber has a variable volume,
- wherein said device also comprises valve devices for allowing or interrupting fluid circulation at least at the first and second inlets and at the at least one collection outlet and the at least one discharge outlet, and
- wherein the first and second preparation chambers include at least one deformable wall comprised of a hyperelastic material.
Dependencies are inferred from the claim text.