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US 12,434,175 B2Granted
Method, system, and filtration unit for the isolation of particles from biological samples
AssigneeYantai Ausbio Laboratories Co., Ltd.
InventorZhaoqiang Wang
Life of this patent
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Abstract
A method and an automated liquid handling system for the isolation of particles from a biological sample are provided. A column, a container and a filtration unit which are adapted to be used in such a method and system are provided. The column can include a section comprising a plurality of microbeads retained there.
Claims14
A method for the isolation of particles from a biological sample, the method comprising:
- providing a column with an upper opening and an elongated channel with a lower opening, the elongated channel comprising at least a first section comprising a first plurality of substantially non-porous microbeads of a first diameter, wherein the first diameter is in a range of 1 μm to 700 μm;
- providing a container that is positioned below the column that comprises a removable plug, the removable plug being positioned at the lower opening to prevent the first plurality of substantially non-porous microbeads from passing through the lower opening, wherein the removable plug comprises a centered protrusion of the container, and wherein at least a portion of the centered protrusion fits into the elongated channel, wherein a passage is formed between lateral walls of the elongated channel and lateral walls of the centered protrusion of the container, wherein the passage has a width allowing a liquid, or a first portion of particles of the biological sample to flow through the passage and into a chamber of the container, and wherein the first section providing interstices between neighboring substantially non-porous microbeads which act as a filter for the biological sample;
- applying the biological sample onto the first section through the upper opening of the column;
- separating the first portion of particles of the biological sample from a second portion of particles of the biological sample, the first portion of particles of the biological sample passing through the first section, the first section retaining the second portion of particles of the biological sample, and the retained second portion of particles being trapped within the interstices;
- suspending the retained second portion of particles of the biological sample and the first section in a buffer solution to form a suspension;
- and
- separating the suspended second portion of particles of the biological sample from the suspended first section.
4 more elements
An automated liquid handling system for the isolation of particles from a biological sample, comprising:
- a column with an upper opening and an elongated channel having a lower opening, the elongated channel comprising at least a first section comprising a first plurality of substantially non-porous microbeads of a first diameter, wherein the first diameter is in a range of 1 μm to 700 μm, the first plurality of substantially non-porous microbeads being retained in the column by a constricting means that is positioned at the lower opening to prevent the first plurality of substantially non-porous microbeads from passing through the lower opening and
- a container,
- wherein the constricting means comprises a removable plug, wherein the removable plug is a centered protrusion of the container and is positioned at the lower opening of the elongated channel, and wherein at least a portion of the protrusion fits into the elongated channel, wherein a passage is formed between lateral walls of the elongated channel and lateral walls of the centered protrusion of the container, wherein the passage has a width allowing a liquid, or a first portion of particles of the biological sample to flow through the passage and into a container chamber,
- and wherein the first section providing interstices between neighboring substantially non-porous microbeads which act as a filter for the biological sample;
- means for separating the first portion of particles of the biological sample from a second portion of particles of the biological sample, the first portion of particles of the biological sample passing through the first section, and the first section retaining the second portion of particles of the biological sample, and the retained second portion of particles being trapped within the interstices;
- means for suspending the retained second portion of particles of the biological sample and the first section in a buffer solution to form a suspension;
- and
- means for separating the suspended second portion of particles of the biological sample from the suspended first section.
5 more elements
Dependencies are inferred from the claim text.