Humanized light chain mice
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLynn Macdonald, Cagan Gurer, Karolina A. Hosiawa, Sean Stevens, Andrew J. Murphy
Life of this patent
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Abstract
Non-human animals, tissues, cells, and genetic material are provided that comprise a modification of an endogenous non-human heavy chain immunoglobulin sequence and that comprise an ADAM6 activity functional in a mouse, wherein the non-human animals express a human immunoglobulin heavy chain variable domain and a cognate human immunoglobulin λ light chain variable domain.
Claims20
A method of making a genetically modified mouse embryonic stem(ES) cell, comprising genetically modifying the mouse ES cell so that its genome comprises:
- (a) one or more human Vλ gene segments and one or more human Jλ gene segments upstream of a mouse immunoglobulin κ light chain constant region (Cκ),
- (b) one or more human VH gene segments, one or more human DH gene segments, and one or more human JH gene segments upstream of a mouse immunoglobulin heavy chain constant region, and
- (c) a nucleotide sequence that encodes a mouse ADAM6 protein, wherein the mouse ADAM6 protein is capable of restoring or enhancing fertility in a genetically modified male mouse.
A genetically modified mouse embryonic stem(ES) cell comprising in its genome:
- (a) one or more human Vλ gene segments and one or more human Jλ gene segments upstream of a mouse immunoglobulin κ light chain constant region (Cκ),
- (b) one or more human VH gene segments, one or more human DH gene segments, and one or more human JH gene segments upstream of a mouse immunoglobulin heavy chain constant region, and
- (c) a nucleotide sequence that encodes a mouse ADAM6 protein, wherein the mouse ADAM6 protein is capable of restoring or enhancing fertility in a genetically modified male mouse.
Dependencies are inferred from the claim text.