Multi-column for isolating exosomes and exosome isolation method

A multi-column for isolating exosomes, the multi-column comprising:

• porous beads (a) having a pore size ranging from 20 nanometers (nm) to 100 nm;
• porous beads (b) having a pore size of 20 nm or less;
• and
• a separation membrane disposed between the porous beads (a) and (b),
• wherein the porous beads (a) are disposed at a lower portion through which an eluent of a sample is discharged, and are configured to separate exosomes and lipoproteins from a biological sample;
• wherein the porous beads (b) are disposed at an upper portion into which the sample is introduced, and are configured to separate exosomes and water-soluble proteins from the biological sample;
• and
• wherein the separation membrane is made of a resin interposed between the porous beads (a) and the porous beads (b) so as to prevent mixing of the porous beads (a) and the porous beads (b).