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US 12,428,666 B2Granted
Compositions and methods related to kethoxal derivatives
AssigneeThe University of Chicago
InventorsChuan He, Xiaocheng Weng, Tong Wu
Life of this patent
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Abstract
Embodiments are directed to N3-kethoxal reagents and derivatives thereof, and related methods that allow fast and reversible labeling of single-stranded nucleic acids in live cells. By way of example, one aspect is directed to a process for reversible labeling of single-stranded guanine bases in live cells, which results in an effective in vivo method for transcriptome-wide RNA secondary structure mapping and RNA G-quadruplex prediction.
Claims15
A compound of the formula:
- wherein Y is an alkyne, an azide, a strained alkyne, a diene, a dieneophile, an alkoxyamine, a phosphine, a hydrazide, a thiol, or an alkene;
- and
- X is a C1 to C10 alkyl or a C4 to C10 polyethylene glycol linker.
A compound of the formula:
- wherein Y is an alkyne, an azide, a strained alkyne, a diene, a dieneophile, an alkoxyamine, a phosphine, a hydrazide, a thiol, or an alkene;
- and X is CH2.
A compound having the formula:
Dependencies are inferred from the claim text.