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US 12,428,662 B2Granted
Methods for making high intensity sweeteners
AssigneeFirmenich SA
InventorsAndrew P. Patron, Chris Edano Noriega, Rama R. Manam, Justin Colquitt, Nathan Faber, Helge Zieler, Justin Stege
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Abstract
Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.
Claims6
A method of producing Compound 1 having the structure of:
- the method comprising:
- contacting mogroside IIIE with an enzyme capable of catalyzing a production of Compound 1 from mogroside IIIE;
- wherein the enzyme is selected from the group consisting of:
- β-glucosidases, transglucosidases,
- a cyclomaltodextrin glucanotransferase (CGTase) comprising an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs:
- 3, 78-82, 85-87, 89-92, 94, 95, 98, 101, and 154, and
- a dextransucrase comprising an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs:
- 106-109, 156, and 896.
5 more elements
Dependencies are inferred from the claim text.