Isotachophoresis for purification of nucleic acids
AssigneePurigen Biosystems, Inc.
InventorsLewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A Rose, Juan G. Santiago
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Abstract
The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.
Claims19
A microfluidic chip comprising two or more fluidic channels each in fluid communication with a sample reservoir configured to receive a sample, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate, wherein the two or more fluidic channels each comprise a branched fluidic channel comprising a loading section and an isotachophoresis (“ITP”) section wherein the ITP section comprises a leading electrolyte branch and an elution branch, meeting at a branch point.
A method comprising:
- introducing a sample into a sample reservoir of a microfluidic chip comprising two or more fluidic channels each in fluid communication with the sample reservoir, a first buffer reservoir configured to receive a buffer, a second buffer reservoir configured to receive a second buffer, a third buffer reservoir configured to receive a third buffer and an elution reservoir configured to receive an eluate, wherein the two or more fluidic channels each comprise a branched fluidic channel comprising a loading section and an isotachophoresis (“ITP”) section wherein the ITP section comprises a leading electrolyte branch and an elution branch, meeting at a branch point.
Dependencies are inferred from the claim text.